PRPHD Kit (Platelet Rich Plasma)

Explanation

PRPHD KIT (PLATELET RICH PLASMA)

PRPHD

WHY PRPHD ?

During the R&D period of PRP HD Kit, we have examined the structures of all other PRP products and we have consulted to our medical doctors. And we have developed safe, effective, injectable, re-suspension featured and quality product along with our doctors qualified experiences.

PRPHD

REAL PRP VITALITY

We are not talking about an IVD Tube, or imported products and packed in bags or boxes. We are talking about our tubes developed for the purpose of PRP applications along with other supportive equipments for safe, sterile and effective applications.

PRPHD

THIS IS NOT IVD

As it is expressed on the table that the IVD Tubes do not require any clinical evaluation process and they are not tested in the frame of biocompatibility. For this reason, IVD tubes are prohibited from clinical applications. This kit is CE marked of Class IIb according to MEDDEV 93/42/EEC.

EASY TO USE, ONLY IN 4 STEPS

HARVESTING THE WHOLE BLOOD

CENTRIFUGATION

HARVESTING THE PRP

RE-SUSPENSION PROCESS

Content of PRPHD Kit

PRPHD TUBES

PRP HD Kit includes 2 PRP HD Tubes in each kit. PRP HD Tube is a 10ml volume, vacuumed tube, includes 1ml of anticoagulant. PRP HD Tube is a sterile tube. The tubes do not include Ficoll or gel. PRP HD Tubes are non-hemolytic and non-pyrogenic. It is recommended that 2 ml of PRP shall be harvested from each PRP HD a Tubes. And totally it can be harvested 4ml of PRP from a single PRP HD Kit.

PRPHD RE-SUSPENSION TUBE

PRP HD Kit includes 1 PRP HD Re-Suspension Tube in each kit. PRP HD RE-Suspension tube is a 5ml volume, plain tube. PRP HD Re-Suspension Tube is a sterile Tube. The tube is non-hemolytic and non-pyrogenic. Total 4 ml of PRP is collected to 5 ml PRP HD Re-suspension tube according to instructions of the manufacturer. Recommended Re-Suspension process is gently shaking the tube for 30 seconds – 1 minute.

INJECTORS & NEEDLES

There are 2 injectors in each kit. One of them is 5ml capacity and the other one is 1 ml capacity sterile injectors. 5 ml injector is used to collect the PRP and to carry the PRP to PRP HD Re-Suspension tube. 1 ml injector is used to apply the PRP to the patient. There are 3 needles in each kit.

A Long Needle, a 21G Needle and a 30G needle. Long needle is used to harvest the PRP from PRP HD Tube and to carry the PRP from PRP HD Tube to PRP HD Re-Suspension Tube. 21G needle is used to harvest the re-suspended PRP inside from the PRP HD Re-Suspension Tube. 30G needle is for injection.

PHLEBOTOMY SET

There is a 2-pieces equipment in the kit. It is used to draw blood from patient into PRP HD Tube. No other equipment is needed to draw the whole blood from the patient.

How to use PRPHD Kit ?

PRPHD

HARVESTING THE WHOLE BLOOD

The whole blood is harvested into PRPHD tube from the patient’s arm using phlebotomy set of PRPHD Kit. Each PRPHD Tube is 10ml volume including 1ml of anticoagulant. Tubes provide easily harvesting of the whole blood as they are vacuumed tubes. Both tubes stop when vacuum is finished. So, you have totally the 18ml of whole blood. After harvesting the whole blood, gently shake both tubes to permeate the anticoagulant into whole blood in the tubes.

PRPHD

CENTRIFUGATION

Each centrifuging unit can be used to perform this process. Tubes shall be put accordingly in order to achieve the centrifuging balance. No special centrifuging device is provided or recommended. The most important subject for centrifuging is to perform 830G for 8 minutes according to instructions for use. Swing rotor centrifugation devices are recommended for more convenient results. Centrifuging devices generally express the RPM (Repeat Per Minute) instead of G force but RPM and G Force can lead to different ratios due to radius difference of the devices. Please check our web page out to convert the RPM into G Force.

PRPHD

HARVESTING THE PRP

This section is the crucial part to obtain the platelet rich plasma. Please do not shake or move the tubes upside down after centrifugation process. After centrifugation you shall see 3 layers in the tube. It means centrifugation process is done properly.

PRPHD

RE-SUSPENSION PROCESS

The PRP collected into 5ml injector is carried to PRPHD Re-Suspension Tube. Re-suspension Tube is plain and sterile. This tube is 5ml volume and totally 4 ml PRP collected from PRPHD Tubes (2ml each as recommended) are placed in this tube. After carrying the PRP into PRPHD Re-suspension tube, gently shake the tube for about 30 seconds to 1 minute to avoid possible clumped cells. This process supports the homogenization of the cells in the plasma milieu. Attach 21G needle to 1ml injector and collect the PRP inside from the PRPHD Re-suspension tube. Change the needle to 30G and you are ready to apply the injection method to the patient.

UNDERSTANDING THE PRP LAYER

Centrifugation process makes the cell layers separated each other according to the molecular weight of the cells. RBC (Red Blood Cell) layer is formed at the bottom of the tube as RBCs are bigger cells in comparison to other component of the whole blood. Right above the RBC layer, there is buffy coat layer with a very small volume (<%1 of the whole blood vol.) where the possible clump formation occurs. Upper Layer is yellowish colored layer. This layer includes PRP and PPP layers together. But those layers can not be recognized with naked eye. PRP is defined as 2ml volume right above the buffy coat layer. Upper part of it is PPP.

HARVESTING THE PRP LAYER

Long needle is attached to 5ml injector and this equipment is put vertically into the tube. The edge of the needle is placed right on the Buffy coat layer and PRP is collected with tornado movement of the needle and injector. 2ml of PRP shall be collected. There is also recommended to collect small amount of RBCs along with Buffy Coat layer and PRP. This is important for the Cell-Cell interaction during the Re-suspension process.